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KMID : 0861020120270020077
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Korea Journal of Herbology
2012 Volume.27 No. 2 p.77 ~ p.83
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Effects of the water extract from Achyranthis Radix on serum-deprivation-induced apoptosis in PC12 cells and transient cerebral middle artery occlusion-induced ischemic brains of rats
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Oh Tae-Woo
Park Ki-Ho
Lee Mi-Young
Choi Go-Ya
Park Yong-Ki
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Abstract
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Objectives: This work was designed to investigate the effect of The root of Achyranthes japonica Nakai (AJN)
water extract on serum deprivation?reperfusion-induced apoptosis in PC-12 cells and transient middle cerebral
artery occlusion (tMCAO)-induced ischemic brains of rats.
Methods: Apoptosis in PC12 cells was induced by serum deprivation and reperfusion. The cells were treated
with AJN water extract at doses of 0.5 and 1.0 ㎎/㎖ for 24 hr after inducing the apoptosis. Cell viability was
determined by WST-1 assay. The expression of caspase-3 protein was determined by Western blot. Ischemic
brains were prepared from tMCAO-induced ischemic rats after oral administration with AJN at dose of 50 and
100 ㎎/㎏, and then brain infarction was measured by TTC staining.
Results: AJN significantly increased the cell viability in apoptocic-induced PC-12 cells, and also decreased the
expression of caspase-3 protein. Furthermore, the administration of AJN significantly inhibited tMCAO-induced
brain infarction in rats.
Conclusions: Our results suggest that AJN extract has a neuroprotective property via suppressing the apoptosis
in PC12 cells and the infarction of ischemic brains.
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KEYWORD
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Achyranthes japonica Nakai, Neuroprotection, anti-apoptotic, PC-12 cell, Cerebral ischemia-reperfusion
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